Journal: Molecules

Article Title: Blackcurrant Extract with Phytoestrogen Activity Alleviates Hair Loss in Ovariectomized Rats

doi: 10.3390/molecules24071272

Figure Lengend Snippet: Figure 5. Induction of K15 and CD34 expression in hair follicles by BCE. Skin from treated rats was stained with anti-K15 antibodies (red signals) or anti-CD34 antibodies (red signals). The nuclei were visualized using 4′,6-diamidino-2-phenylindole. * Autofluorescence from the hair shaft. Scale bar = 100 μm.

Article Snippet: Subsequently, the slides were incubated with anti-cytokeratin 19 (A-3) antibodies (cat. no. sc-376126; Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:50 dilution, v/v), anti-cytokeratin 15 (LHK15) antibodies (cat. no. sc-47697; Santa Cruz Biotechnology; 1:50 dilution, v/v), or anti-CD34 (B-6) antibodies (cat. no. sc74499; Santa Cruz Biotechnology; 1:50 dilution, v/v) at 4 °C overnight.

Techniques: Expressing, Staining

Journal: Journal of Nanobiotechnology

Article Title: Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration

doi: 10.1186/s12951-023-02125-5

Figure Lengend Snippet: Characterization of hUCMSCs. A Primary hUCMSCs were spindle-shaped under light microscopy. B Flow cytometry analysis showed that 98.5% of cells expressed CD29, 97.4% expressed CD44, 98.6% of cells expressed CD90, and 98.1% of cells expressed CD105. Meanwhile, only 0.48% and 0.28% expressed CD45 and CD34. C Lipid droplets were stained by oil red O after adipogenic differentiation induction of hUCMSCs. Mineralized nodules were stained by Alizarin Red after osteogenic differentiation induction of hUCMSCs. Acid mucopolysaccharide was stained by Alcian Blue after chondrogenic differentiation induction of hUCMSCs

Article Snippet: Briefly, 10 7 hUCMSCs were subjected to analysis for surface markers CD34 (E-AB-F1143D, Elabscience), CD44 (E-AB-F1100D, Elabscience), CD45 (E-AB-F1137D, Elabscience), CD29 (E-AB-F1049D, Elabscience), CD90 (E-AB-F1167D, Elabscience), and CD105 (E-AB-F1310D, Elabscience) by flow cytometry following the manufacturer’s protocol.

Techniques: Light Microscopy, Flow Cytometry, Staining

Journal: Stem Cell Research & Therapy

Article Title: Growth differentiation factor 11 alleviates oxidative stress-induced senescence of endothelial progenitor cells via activating autophagy

doi: 10.1186/s13287-024-03975-y

Figure Lengend Snippet: Rat bone marrow-derived CD34 + VEGFR-2 + EPCs. ( A ) Analysis of CD34 + VEGFR-2 + EPCs by flow cytometry. ( B ) CD34 + VEGFR-2 + EPCs (arrows) in the mononuclear cells isolated from bone marrow. Immunostaining. Scale bar = 25 μm. ( C ) CD31 expression in CD34 + VEGFR-2 + EPCs at 2 weeks after induction with VEGF. Immunostaining. Scale bar = 10 μm. ( D , E ) ALK-4 expression in EPCs demonstrated with immunostaining and Western blotting respectively. Scale bar = 25 μm. ( F ) The statistical result of ALK-4 expression. * P < 0.01 versus young group. n = 3

Article Snippet: The cells were then incubated with mouse anti-rat CD34 and rabbit anti-rat VEGFR-2 antibodies (1:100; Santa Cruz Biotech, Dallas, TX, USA) at 4 o C for 50 min, followed by incubation with Alexa Fluor 647-conjugated goat anti-mouse IgG and Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200; Jackson, West Grove, PA, USA) at 4 o C for 30 min. CD34 + VEGFR-2 + cells in the mononuclear cells were sorted using a Beckman MoFloTM XDP FACS (fluorescence-activated cell sorter; Beckman Coulter, Fullerton, CA, USA).

Techniques: Derivative Assay, Flow Cytometry, Isolation, Immunostaining, Expressing, Western Blot